Preprint / Version 1

Throwing cold water on muscle growth

A systematic review with meta-analysis of the effects of post-exercise cold water immersion on resistance training-induced hypertrophy

##article.authors##

  • Alec Piñero
  • Ryan Burke
  • Francesca Augustin
  • Adam Mohan
  • Kareen Dejesus
  • Max Sapuppo
  • Max Weisenthal
  • Max Coleman
  • Patroklos Androulakis-Korakakis
  • Jozo Grgic
  • Paul Swinton
  • Brad Schoenfeld Lehman College

DOI:

https://doi.org/10.51224/SRXIV.301

Keywords:

recovery strategies, cold application, cooling, cross-sectional area, fat-free mass, lean mass

Abstract

The purpose of this paper was to systematically review the literature and perform a meta-analysis of the existing data on the effects of post-exercise cooling coupled with resistance training (RT) on gains in measures of muscle growth. To locate relevant studies for the topic, we comprehensively searched the PubMed/MEDLINE, Scopus, and Web of Science databases. A total of 8 studies met inclusion criteria; all employed cold water immersion (CWI) as the means of cold application. Preliminary analyses conducted on non-controlled effect sizes provided strong evidence of hypertrophic adaptations with RT that were likely to be at least small in magnitude (SMD0.5 = 0.36 [95%CrI: 0.10 to 0.61]; p(>0) = 0.995, p(>0.1) = 0.977). In contrast, non-controlled effect sizes provided some evidence of hypertrophic adaptations with CWI + RT that were likely to be between small and zero in magnitude (SMD0.5 = 0.14 [95%CrI: -0.08 to 0.36]; p(>0) = 0.906, p(>0.1) = 0.68). The primary analysis conducted on comparative effect sizes provided some evidence of greater relative hypertrophic adaptations with RT compared to CWI + RT (cSMD0.5 = -0.22 [95%CrI: -0.47 to 0.04]), with differences likely to be greater than zero (p(<0) = 0.957) and of at least a small magnitude of effect (p(< -0.1) = 0.834). Meta-regression did not indicate a potential moderation effect of training status (= -0.10 [95%CrI: -0.65 to 0.43] =0.653). In conclusion, the current data suggest that the application of CWI immediately following bouts of RT may attenuate hypertrophic changes.

Metrics

Metrics Loading ...

References

References

Crystal NJ, Townson DH, Cook SB, LaRoche DP. Effect of cryotherapy on muscle recovery and inflammation following a bout of damaging exercise. Eur J Appl Physiol. 2013 October 01;113(10):2577-86.

Wang Y, Li S, Zhang Y, Chen Y, Yan F, Han L, et al. Heat and cold therapy reduce pain in patients with delayed onset muscle soreness: A systematic review and meta-analysis of 32 randomized controlled trials. Phys Ther Sport. 2021 March 01;48:177-87.

Xiao F, Kabachkova AV, Jiao L, Zhao H, Kapilevich LV. Effects of cold water immersion after exercise on fatigue recovery and exercise performance--meta analysis. Front Physiol. 2023 January 20;14:1006512.

Roberts LA, Nosaka K, Coombes JS, Peake JM. Cold water immersion enhances recovery of submaximal muscle function after resistance exercise. Am J Physiol Regul Integr Comp Physiol. 2014 October 15;307(8):R998-R1008.

McPhee JS, Lightfoot AP. Post-exercise recovery regimes: Blowing hot and cold. J Physiol. 2017 February 01;595(3):627-8.

Thorpe RT. Post-exercise recovery: Cooling and heating, a periodized approach. Front Sports Act Living. 2021 September 01;3:707503.

Peake JM, Roberts LA, Figueiredo VC, Egner I, Krog S, Aas SN, et al. The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise. J Physiol. 2017 February 01;595(3):695-711.

Tseng C, Lee J, Tsai Y, Lee S, Kao C, Liu T, et al. Topical cooling (icing) delays recovery from eccentric exercise-induced muscle damage. J Strength Cond Res. 2013 May 01;27(5):1354-61.

Broatch JR, Petersen A, Bishop DJ. The influence of post-exercise cold-water immersion on adaptive responses to exercise: A review of the literature. Sports Med. 2018 June 01;48(6):1369-87.

Versey NG, Halson SL, Dawson BT. Water immersion recovery for athletes: Effect on exercise performance and practical recommendations. Sports Med. 2013 November 01;43(11):1101-30.

Pointon M, Duffield R. Cold water immersion recovery after simulated collision sport exercise. Med Sci Sports Exerc. 2012 February 01;44(2):206-16.

Ihsan M, Abbiss CR, Allan R. Adaptations to post-exercise cold water immersion: Friend, foe, or futile? Front Sports Act Living. 2021 July 16;3:714148.

Chaillou T, Treigyte V, Mosely S, Brazaitis M, Venckunas T, Cheng AJ. Functional impact of post-exercise cooling and heating on recovery and training adaptations: Application to resistance, endurance, and sprint exercise. Sports Med Open. 2022 March 07;8(1):37-022.

Malta ES, Dutra YM, Broatch JR, Bishop DJ, Zagatto AM. The effects of regular cold-water immersion use on training-induced changes in strength and endurance performance: A systematic review with meta-analysis. Sports Med. 2021 January 01;51(1):161-74.

Petersen AC, Fyfe JJ. Post-exercise cold water immersion effects on physiological adaptations to resistance training and the underlying mechanisms in skeletal muscle: A narrative review. Front Sports Act Living. 2021 April 08;3:660291.

Grgic J. Effects of post-exercise cold-water immersion on resistance training-induced gains in muscular strength: A meta-analysis. Eur J Sport Sci. 2023 March 01;23(3):372-80.

Fyfe JJ, Broatch JR, Trewin AJ, Hanson ED, Argus CK, Garnham AP, et al. Cold water immersion attenuates anabolic signaling and skeletal muscle fiber hypertrophy, but not strength gain, following whole-body resistance training. J Appl Physiol (1985). 2019 November 01;127(5):1403-18.

Figueiredo VC, Roberts LA, Markworth JF, Barnett MP, Coombes JS, Raastad T, et al. Impact of resistance exercise on ribosome biogenesis is acutely regulated by post-exercise recovery strategies. Physiol Rep. 2016 February 01;4(2):10.14814/phy2.12670.

Fuchs CJ, Kouw IWK, Churchward-Venne TA, Smeets JSJ, Senden JM, Lichtenbelt WDvM, et al. Postexercise cooling impairs muscle protein synthesis rates in recreational athletes. J Physiol. 2020 February 01;598(4):755-72.

Roberts LA, Raastad T, Markworth JF, Figueiredo VC, Egner IM, Shield A, et al. Post-exercise cold water immersion attenuates acute anabolic signalling and long-term adaptations in muscle to strength training. J Physiol. 2015 September 15;593(18):4285-301.

Earp JE, Hatfield DL, Sherman A, Lee EC, Kraemer WJ. Cold-water immersion blunts and delays increases in circulating testosterone and cytokines post-resistance exercise. Eur J Appl Physiol. 2019 August 01;119(8):1901-7.

Koh TJ, Pizza FX. Do inflammatory cells influence skeletal muscle hypertrophy? Front Biosci (Elite Ed). 2009 June 01;1:60-71.

Ogasawara R, Suginohara T. Rapamycin-insensitive mechanistic target of rapamycin regulates basal and resistance exercise-induced muscle protein synthesis. FASEB J. 2018 May 14:fj201701422R.

Kraemer WJ, Ratamess NA. Hormonal responses and adaptations to resistance exercise and training. Sports Med. 2005;35(4):339-61.

Figueiredo VC, McCarthy JJ. Regulation of ribosome biogenesis in skeletal muscle hypertrophy. Physiology (Bethesda). 2019 January 01;34(1):30-42.

Crewther B, Keogh J, Cronin J, Cook C. Possible stimuli for strength and power adaptation: Acute hormonal responses. Sports Med. 2006;36(3):215-38.

Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. BMJ. 2021 March 29;372:n71.

Greenhalgh T, Peacock R. Effectiveness and efficiency of search methods in systematic reviews of complex evidence: Audit of primary sources. BMJ. 2005 November 05;331(7524):1064-5.

Cooper H, Hedges L, Valentine J. The handbook of research synthesis and meta-analysis. . 2nd ed. New York: Russell Sage Foundation; 2009.

Downs SH, Black N. The feasibility of creating a checklist for the assessment of the methodological quality both of randomised and non-randomised studies of health care interventions. J Epidemiol Community Health. 1998 June 01;52(6):377-84.

Resnick B, Zimmerman SI, Orwig D, Furstenberg AL, Magaziner J. Outcome expectations for exercise scale: Utility and psychometrics. J Gerontol B Psychol Sci Soc Sci. 2000 November 01;55(6):S352-6.

Kruschke JK, Liddell TM. The bayesian new statistics: Hypothesis testing, estimation, meta-analysis, and power analysis from a bayesian perspective. Psychon Bull Rev. 2018 February 01;25(1):178-206.

Morris B. Estimating effect sizes from pretest-posttest-control group designs. Organizational Research Methods. 2008;11(2):364-86.

Morris SB, DeShon RP. Combining effect size estimates in meta-analysis with repeated measures and independent-groups designs. Psychol Methods. 2002 March 01;7(1):105-25.

Madeyski L, Kitchenham B. In: Effect sizes and their variance for AB/BA crossover design studies. Proceedings of the 40th international conference on software engineering; Gothenburg, Sweden. New York, NY: Association for Computing Machinery; May 27 2018. p. 420.

Swinton PA, Murphy A. Comparative effect size distributions in strength and conditioning and implications for future research: A meta-analysis. SportRxiv. 2022:DOI: 10.51224/SRXIV.202.

Swinton PA, Burgess K, Hall A, Greig L, Psyllas J, Aspe R, et al. Interpreting magnitude of change in strength and conditioning: Effect size selection, threshold values and bayesian updating. J Sports Sci. 2022 September 01;40(18):2047-54.

Fu R, Gartlehner G, Grant M, Shamliyan T, Sedrakyan A, Wilt TJ, et al. Conducting quantitative synthesis when comparing medical interventions: AHRQ and the effective health care program. J Clin Epidemiol. 2011 November 01;64(11):1187-97.

Fernandez-Castilla B, Declercq L, Jamshidi L, Beretvas S, Onghena P, Van den Noorthgate W. Detecting selection bias in meta-analyses with multiple outcomes: A simulation study

. J Exp Educ. 2021;89(1):125-44.

Bürkner P. Brms: An R package for bayesian multilevel models using stan. J Stat Softw. 2017;80(1):1-28.

Wilson LJ, Dimitriou L, Hills FA, Gondek MB, van Wyk A, Turek V, et al. Cold water immersion offers no functional or perceptual benefit compared to a sham intervention during a resistance training program. J Strength Cond Res. 2021 October 01;35(10):2720-7.

Horgan BG, Halson SL, Drinkwater EJ, West NP, Tee N, Alcock RD, et al. No effect of repeated post-resistance exercise cold or hot water immersion on in-season body composition and performance responses in academy rugby players: A randomised controlled cross-over design. Eur J Appl Physiol. 2023 February 01;123(2):351-9.

Yamane M, Teruya H, Nakano M, Ogai R, Ohnishi N, Kosaka M. Post-exercise leg and forearm flexor muscle cooling in humans attenuates endurance and resistance training effects on muscle performance and on circulatory adaptation. Eur J Appl Physiol. 2006 March 01;96(5):572-80.

Ohnishi N, Yamane M, Uchiyama N, Shirasawa S, Kosaka M, Shiono H. Adaptive changes in muscular performance and circulation by resistance training with regular cold application. J Thermal Biol. 2004;29:839-43.

Yamane M, Ohnishi N, Matsumoto T. Does regular post-exercise cold application attenuate trained muscle adaptation? Int J Sports Med. 2015 July 01;36(8):647-53.

Poppendieck W, Wegmann M, Hecksteden A, Darup A, Schimpchen J, Skorski S, et al. Does cold-water immersion after strength training attenuate training adaptation? Int J Sports Physiol Perform. 2021 February 01;16(2):304-10.

Brook MS, Wilkinson DJ, Smith K, Atherton PJ. It's not just about protein turnover: The role of ribosomal biogenesis and satellite cells in the regulation of skeletal muscle hypertrophy. Eur J Sport Sci. 2019 August 01;19(7):952-63.

Uchiyama S, Tsukamoto H, Yoshimura S, Tamaki T. Relationship between oxidative stress in muscle tissue and weight-lifting-induced muscle damage. Pflugers Arch. Invalid date Invalid date;452(1):109-16. Available from: http://www.hubmed.org/display.cgi?uids=16402246.

Roux PP, Blenis J. ERK and p38 MAPK-activated protein kinases: A family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev. 2004 June 01;68(2):320-44.

Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N. Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol. Invalid date Invalid date;88(1):61-5. Available from: http://www.hubmed.org/display.cgi?uids=10642363.

Ahokas EK, Kyröläinen H, Mero AA, Walker S, Hanstock HG, Ihalainen JK. Water immersion methods do not alter muscle damage and inflammation biomarkers after high-intensity sprinting and jumping exercise. Eur J Appl Physiol. 2020 December 01;120(12):2625-34.

Timmerman KL, Lee JL, Dreyer HC, Dhanani S, Glynn EL, Fry CS, et al. Insulin stimulates human skeletal muscle protein synthesis via an indirect mechanism involving endothelial-dependent vasodilation and mammalian target of rapamycin complex 1 signaling. J Clin Endocrinol Metab. 2010 August 01;95(8):3848-57.

Fujita S, Rasmussen BB, Cadenas JG, Grady JJ, Volpi E. Effect of insulin on human skeletal muscle protein synthesis is modulated by insulin-induced changes in muscle blood flow and amino acid availability. Am J Physiol Endocrinol Metab. 2006 October 01;291(4):E745-54.

Gregson W, Black MA, Jones H, Milson J, Morton J, Dawson B, et al. Influence of cold water immersion on limb and cutaneous blood flow at rest. Am J Sports Med. 2011 June 01;39(6):1316-23.

Mawhinney C, Jones H, Low DA, Green DJ, Howatson G, Gregson W. Influence of cold-water immersion on limb blood flow after resistance exercise. Eur J Sport Sci. 2017 June 01;17(5):519-29.

Schoenfeld BJ, Aragon AA. Is there a postworkout anabolic window of opportunity for nutrient consumption? clearing up controversies. J Orthop Sports Phys Ther. 2018 December 01;48(12):911-4.

Schoenfeld BJ. Postexercise hypertrophic adaptations: A reexamination of the hormone hypothesis and its applicability to resistance training program design. J Strength Cond Res. 2013 June 01;27(6):1720-30.

Haun CT, Vann CG, Roberts BM, Vigotsky AD, Schoenfeld BJ, Roberts MD. A critical evaluation of the biological construct skeletal muscle hypertrophy: Size matters but so does the measurement. Front Physiol. 2019 March 12;10:247.

Delmonico MJ, Kostek MC, Johns J, Hurley BF, Conway JM. Can dual energy X-ray absorptiometry provide a valid assessment of changes in thigh muscle mass with strength training in older adults? Eur J Clin Nutr. 2008 December 01;62(12):1372-8.

Levine JA, Abboud L, Barry M, Reed JE, Sheedy PF, Jensen MD. Measuring leg muscle and fat mass in humans: Comparison of CT and dual-energy X-ray absorptiometry. J Appl Physiol. 2000 February 01;88(2):452-6.

Horwath O, Envall H, Röja J, Emanuelsson EB, Sanz G, Ekblom B, et al. Variability in vastus lateralis fiber type distribution, fiber size, and myonuclear content along and between the legs. J Appl Physiol (1985). 2021 July 01;131(1):158-73.

Aragon AA, Schoenfeld BJ. Magnitude and composition of the energy surplus for maximizing muscle hypertrophy: Implications for bodybuilding and physique athletes. Strength & Conditioning Journal. 2020;42(5):79-86.

Morton RW, Murphy KT, McKellar SR, Schoenfeld BJ, Henselmans M, Helms E, et al. A systematic review, meta-analysis and meta-regression of the effect of protein supplementation on resistance training-induced gains in muscle mass and strength in healthy adults. Br J Sports Med. 2017 July 11.

Baláš J, Kodejška J, Krupková D, Giles D. Males benefit more from cold water immersion during repeated handgrip contractions than females despite similar oxygen kinetics. J Physiol Sci. 2020 March 05;70(1):13-020.

Castellani JW, Young AJ. Human physiological responses to cold exposure: Acute responses and acclimatization to prolonged exposure. Auton Neurosci. 2016 April 01;196:63-74.

Petrofsky JS, Laymon M. Heat transfer to deep tissue: The effect of body fat and heating modality. J Med Eng Technol. 2009;33(5):337-48.

DeFreitas JM, Beck TW, Stock MS, Dillon MA, Kasishke PR. An examination of the time course of training-induced skeletal muscle hypertrophy. Eur J Appl Physiol. 2011 November 01;111(11):2785-90.

Downloads

Posted

2023-06-09